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Flow Cytometry White Papers

A Deeper Dive into Flow Cytometry Data

In recent years, flow cytometry has been advanced by innovations in high-resolution optical analyses of novel fluorochromes. As a result, an increasing number of cellular parameters can now be acquired from limited clinical sample volumes. Various approaches to mining this high-dimensional data are available that extend beyond traditional hierarchical gating and t-distributed stochastic neighbor embedding (t-SNE) to visualize subpopulations. One such approach from AstroLabe, utilizes Multi-dimensional Scaling (MDS) algorithms to identify statistically significant trends across cohorts. FlowMetric is proud to be offering this analytical service to our clients to help maximize the potential of their flow cytometry data.

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Functional Proteomics Provides Powerful Insights Into the Mechanisms and Pathways of T-cell Activation.

In vitro T-cell activation is an important means of immune-profiling for a variety of research and clinical applications. In this study, we use the Isoplexis Functional Proteomics system to evaluate T-cell activation in vitro using two different methods: PMA + Ionomycin and anti-CD3/CD28. Comparing the robustness and diversity of the cytokine responses reveals the need for fit-for-purpose experimental design and highlights the capability of this platform to interrogate T-cell subsets in a unique and powerful way.

To learn more about T-cell Functional Profiling using the Isoplexis platform, download our white paper.

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Cytokine Analysis Using IsoPlexis™ CodePlex™ Reveals Functional Proteomic Differences in COVID-19 Patient Samples

IsoPlexis proteomic technology enables researchers to obtain highly multiplexed cytokine profiles from a variety of clinical sample types. The CodePlex Secretome system performs automated, multiplexed proteomic evaluation of 30+ secreted functional and inflammatory cytokine markers, to provide insight into early predictive markers of inflammatory responses.

To learn more about the role of Cytokine Release Syndrome (CRS) in COVID-19 infections, download our white paper.

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T Cell Exhaustion in Immuno-oncology: Understanding this Delicate Balancing Act

We’ve entered an age of unparalleled leaps in immune-based therapies and personalized medicine to treat a large variety of cancers.

Our understanding of the molecular mechanisms associated with T cell exhaustion have been crucial in developing these therapies, but this research has also uncovered some unexpected consequences to reversing exhaustion.

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Introduction To Ploidy

Ploidy represents the number of complete chromosome sets in a cell’s nucleus. The generation of gametes, or germ cells by meiosis, leads to haploidy, with half of the normal number of chromosomes.

Polyploidy is a condition where the nuclei carries three or more times the number of chromosomes found in haploid cells. This condition is common in plants and believed to provide hybrid vigor and defend against potentially destructive recessive mutations.

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Computational Cytometry: Flow Cytometry Data Analysis in the Era of Quantitative Data Science

Flow cytometry has become a widely-used biomedical research technique because it can characterize and measure numerous cell types.

We’ve entered an era in which flow cytometers can routinely measure 12 different cell parameters and beyond. Advances in hardware have been coupled with a change in how flow cytometry data has been analyzed, and now most flow cytometry users are discovering how computational analysis can help them better understand and visualize large datasets.

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Accelerated CAR T Cell Therapy with Flow Cytometry

Immuno-oncology has transformed the way many cancers are treated through customized cell therapies. One specific therapy uses T cells that carry chimeric antigen receptors (CAR T cells) that can treat hematologic malignancies and solid tumors.

Flow cytometry has been a crucial part of the preclinical development and clinical monitoring of these cells since these studies first began.

Learn more about how flow cytometry has been used in this developing and implementing this experimental therapy.

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Flow Cytometry for Food Science and Agricultural Applications

Flow cytometry protocols are being used more frequently for food science and agricultural applications, and have been developed into validated assays that can meet the needs of industry scientists.

This white paper features several alternative flow cytometry applications in food science and agriculture that can meet the demand for rapid, precise, and reproducible results in fields highly reliant on accurate cell counts and downstream single cell analysis techniques.

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Using Fluorescence Activated Cell Sorting in Preclinical and Clinical Research

Many researchers across biomedical disciplines face a situation when they need to isolate a pure population of cells. They might be stem cells for basic research or a patient’s T cells that can be engineered to attack their own tumor. The many uses for pure cell populations in both the preclinical and clinical research has created a demand for techniques that preserve the viability and functionality of cells and optimize the purity of the cell population of interest. Fluorescence activated cell sorting (FACS) is a flow cytometry-based technique that is the workhorse of biomedical research because it can satisfy these criteria.

This white paper highlights the many applications of FACS in both basic and clinical research, and how cell sorting techniques can be customized for the specific downstream uses of the sorted cells.

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Flow Cytometry & Immunology Research - A Powerful Tool For Cutting Edge Basic And Translational Research

Flow cytometry applications span the immunology field and have been used to advance our understanding of immunological mechanisms and develop novel treatments for cancer, infectious diseases, and autoimmune diseases.

This white paper highlights novel uses of flow cytometry in different immunology subfields and how this tool has advanced our scientific knowledge.

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Safety and Toxicity Studies Using Flow Cytometry - For GLP and non-GLP Compliant Applications

Immunomodulatory drug candidates typically undergo safety and toxicology analysis to assess suitability for human use. At present, flow cytometry-based safety and toxicology assays are used in tandem with traditional assays and can be performed under good laboratory practice (GLP) conditions or can be used for non-GLP safety and toxicology assessments.

This white paper provides an overview of how flow cytometry-based assays can be used to your safety and toxicity analysis and provide you with information critical to informing preclinical development decisions.

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Functional Assays by Flow Cytometry

Immune system cells have evolved to execute numerous functions that defend the host against pathogens and other threats like tumors. Scientists have worked for decades to identify and measure cellular function and numerous experimental protocols have been developed to this end. In today’s laboratory, flow cytometry has emerged as a precise, rapid, and customizable platform for many state-of-the-art functional assays.

This white paper provides a summary of flow cytometry functional assays currently being used in basic and clinical research settings.

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Flow Cytometry for Clinical Trials: Assay Development, Validation, Harmonization, and Clinical Data Acquisition

Flow cytometry can measure multiple cellular characteristics simultaneously, which makes it an “assay of choice” for profiling cell phenotypes or measuring production of immune molecules, and it can also be used to characterize signature biomarkers in a variety of situations. Unlike basic research, flow cytometry assays for clinical trials must fulfill regulatory requirements including instrument and method validation. If you are considering using a flow cytometry assay for your next pre-clinical research project or clinical trial, consider consulting with an expert in clinical flow cytometry as you plan your project.

This white paper gives an overview of points to consider when implementing a clinical trial flow cytometry assay from project inception to data collection.

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Immuno-Oncology Research and Flow Cytometry - Transforming Cancer Treatment in the 21st Century

Cancer treatment is undergoing a revolution. Decades of basic and clinical research have revealed how the immune system can be harnessed to destroy tumors specifically or prevent metastasis. Along the way, flow cytometry has been a powerful tool for determining the mechanism of action of certain therapeutic treatments, monitoring responses in clinical trials, and tracking the effectiveness of treatment in patients.

To learn more about how Flow Cytometry can be used in Immuno-Oncology Research, download our white paper.

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Outsourcing with CRO's - Flow Cytometry and Beyond

A CRO can carry out your experiments, to your exact specifications, and meet your needs for a single experiment or a multi-year clinical trial. CROs specializing in specific assay types or techniques may be your best option to guarantee high quality and reliable results.

In this white paper, we describe how to establish and reap the benefits of a successful Contract Research Organization (CRO) relationship.

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Know Your Flow - Five Rules for Optimal Panel Design

Flow cytometry panel design has transformed biomedical research, especially in the fields of immunology, hematology, oncology, and genetics. Cells are at the heart of each flow cytometry experiment and are stained with a panel of fluorochrome-labeled antibodies to reveal characteristics about individual cells and the entire cell population.

In this white paper, we describe five basic rules that can help flow cytometry users get a better handle on panel design.

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Considering the Full Picture: Genomics, Proteomics, and Flow Cytometry

In the current world of data-dense science, we have grown accustomed to having detailed data about individual cells for both basic research and clinical applications. What is this data and how is it obtained rapidly and accurately? The current decade has brought breakthroughs in a wide range of -omics: genomics, proteomics, transcriptomics and more.

This technical brief will explain the concepts behind each of these technologies and how they are critical to advancing preclinical and clinical research.

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FlowMetric PhosphoFlow Case Study: Cell Signaling Assays

Objective: In order to better assess cell functionality, FlowMetric was tasked to analyze key phosphoproteins in lymphocyte subsets without prior isolation from whole blood patient donor samples.

Read more about this phosphoflow-oriented case study.

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Improve Your Preclinical Prospects - Using Flow Cytometry in Preclinical Development

How do you find the next great treatment or medication? Many new pharmaceutical drugs and biologics in development or being FDA approved harness the power of the immune system. These therapies treat cancer, autoimmune diseases, and infections, but identifying high quality drug candidates and understanding their mechanism of action (MOA) requires using techniques that reveal the inner workings of the immune system. Flow cytometry is that unparalleled technique.

To learn more about how Flow Cytometry can be use throughout Preclinical development, download our white paper.

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